Sangam: A Confluence of Knowledge Streams

Lipopolysaccharide Pretreatment of Cyclosporine-Treated Rats Enhances Cardiac Allograft Survival

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dc.contributor Section of Thoracic Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109
dc.contributor Section of Thoracic Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109
dc.contributor Section of Thoracic Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109
dc.creator Lin, M. D. , Hua
dc.creator Wei, Ru-Qi
dc.creator Bolling, M. D. , Steven F.
dc.date 2006-04-10T15:35:00Z
dc.date 2006-04-10T15:35:00Z
dc.date 1993-10
dc.date.accessioned 2022-05-19T11:30:55Z
dc.date.available 2022-05-19T11:30:55Z
dc.identifier Lin, M.D., Hua, Wei, M.D., Ru-Qi, Bolling, M.D., Steven F. (1993/10)."Lipopolysaccharide Pretreatment of Cyclosporine-Treated Rats Enhances Cardiac Allograft Survival." Journal of Surgical Research 55(4): 441-445. <http://hdl.handle.net/2027.42/30558>
dc.identifier http://www.sciencedirect.com/science/article/B6WM6-45P66VJ-12/2/68e9e73b093e78c44dbd0d6af691bda5
dc.identifier http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8412131&dopt=citation
dc.identifier http://hdl.handle.net/2027.42/30558
dc.identifier 8412131
dc.identifier http://dx.doi.org/10.1006/jsre.1993.1166
dc.identifier Journal of Surgical Research
dc.identifier.uri http://localhost:8080/xmlui/handle/CUHPOERS/105193
dc.description Lipopolysaccharide (LPS), the endotoxin in gram-negative bacterial cell walls, is a major factor in septic shock. Tumor necrosis factor (TNF) appears immediately after LPS release or LPS injection in rats, but when these animals have LPS reinjected for up to 7 days, TNF production is inhibited. Because inhibiting TNF with anti-TNF antibodies prolongs cardiac allograft survival and is synergistic with cyclosporine (CsA), enhanced graft survival could result from inhibiting TNF via LPS pretreatment. Accordingly, heterotopic rat heart transplants were performed in: I, untreated controls: II, LPS pretransplant treatment: III, LPS post-transplant treatment; IV, low-dose CsA posttransplant treatment; V, CsA post-transplant treatment and PBS (LPS vehicle); or VI, LPS pretransplant treatment and low-dose CsA post-transplant treatment, using Brown Norway (BN) donors and Lewis (LEW) recipients. Rejection was defined by a lack of contractions. Results showed that while LPS pre- or post-treatment alone had little allograft survival effect, LPS pretreatment combined with CsA significantly prolonged survival vs control or CsA alone (22.0 +/- 1.6 days vs 6.8 +/- 0.6 days or 13.4 +/- 1.1 days; P 3H]thymidine incorporation than untreated LEW splenocytes (3671 +/- 349 vs 7828 +/- 14 cpm). TNF assays of untreated and PBS-treated LEW spleen cells cocultured with irradiated BN spleen cells had 1.3 and 1.1 pg of TNF/106 cells, respectively, in 2 hr, but no TNF from LPS-pretreated LEW cells was detected. These results suggest that LPS-enhanced allograft survival may be due to TNF inhibition and lymphocyte suppression, providing insight into immunosuppressive mechanisms.
dc.description Peer Reviewed
dc.description http://deepblue.lib.umich.edu/bitstream/2027.42/30558/1/0000191.pdf
dc.format 334377 bytes
dc.format 3118 bytes
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dc.language en_US
dc.publisher Elsevier
dc.rights IndexNoFollow
dc.subject Surgery and Anesthesiology
dc.subject Health Sciences
dc.title Lipopolysaccharide Pretreatment of Cyclosporine-Treated Rats Enhances Cardiac Allograft Survival
dc.type Article


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