1. 1.An electrophoretic method for the rapid separation of the phosphate esters of inositol is presented.2. 2.It is used, together with ion-exchange chromatography, to establish the presence of inositol di- and triphosphates in hydrolysates of trichloroacetic acid residues of rat brain, liver, heart, kidney, and lung.3. 3.With the aid of a 32P marker, inositol di- and triphosphates were also found in hydrolysates of rat erythrocyte stroma.4. 4.Glycerol diphosphate was found in hydrolysates of rat liver.5. 5.Inositol tetraphosphate was not found in the hydrolysates of any of the tissues studied.6. 6.Residues remaining after extraction of rat brain by established lipid extraction procedures retained substantial amounts of bound inositol di- and triphosphates.
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