| dc.creator |
Wang, Hong-yan |
|
| dc.creator |
Lin, Li |
|
| dc.creator |
Fu, Wei |
|
| dc.creator |
Yu, Hui-Yuan |
|
| dc.creator |
Yu, Ning |
|
| dc.creator |
Tan, Li-si |
|
| dc.creator |
Cheng, Jya-wei |
|
| dc.creator |
Pan, Ya-ping |
|
| dc.date |
2017-09-03T05:39:36Z |
|
| dc.date |
2017-09-03T05:39:36Z |
|
| dc.date |
2017-08-29 |
|
| dc.date |
2017-09-03T05:39:39Z |
|
| dc.date.accessioned |
2022-05-19T13:29:57Z |
|
| dc.date.available |
2022-05-19T13:29:57Z |
|
| dc.identifier |
BMC Complementary and Alternative Medicine. 2017 Aug 29;17(1):426 |
|
| dc.identifier |
http://dx.doi.org/10.1186/s12906-017-1931-9 |
|
| dc.identifier |
http://hdl.handle.net/2027.42/138096 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/117308 |
|
| dc.description |
Abstract
Background
P-113 (AKRHHGYKRKFH-NH2) is a 12-amino-acid histidine-rich peptide derived from histatin 5 that is highly degradable in high salt concentrations and biological fluids such as serum, plasma and saliva. Nal-P-113, a novel antimicrobial peptide whose histidine residues are replaced by the bulky amino acids β-naphthylalanine, causes the antimicrobial peptide to retain its bactericidal activity even in physiological environments. This study evaluated the effect of the novel antimicrobial peptide Nal-P-113 in a rat periodontitis model and the mechanisms of action of Nal-P-113 for suppressing periodontitis.
Methods
Periodontitis was induced in mandibular first molars in rats receiving a ligature and infected with Porphyromonas gingivalis. Animals were randomly divided into six groups: a, P. gingivalis W83 alone; b, P. gingivalis W83 with 6.25 μg/mL of Nal-P-113; c, P. gingivalis W83 with 25 μg/mL of Nal-P-113; d, P. gingivalis W83 with 100 μg/mL of Nal-P-113; e, P. gingivalis W83 with 400 μg/mL of Nal-P-113; and f, control without P. gingivalis W83 or Nal-P-113. Morphometric analysis was used to evaluate alveolar bone loss. Microbiological assessment of the presence of Porphyromonas gingivalis and total bacteria was performed using absolute quantitative real-time PCR and scanning electron microscopy. Gingival tissue was collected for western blot and immunohistochemical assays of IL-1β and TNF-α levels.
Results
Alveolar bone loss was inhibited by 100 μg/mL or 400 μg/mL of Nal-P-113 compared to the control group (P < 0.05). Lower amounts of P. gingivalis and total bacteria were found in groups d and e compared with group a (P < 0.05). A decrease in the levels of IL-1β and TNF-α was detected in group d and group e compared to the control group (P < 0.05). The amount of P. gingivalis was positively correlated with IL-1β and TNF-α expression in periodontal tissue (P < 0.05).
Conclusions
Nal-P-113 exhibited protective effects on Porphyromonas gingivalis-induced periodontitis in rats by limiting the amount of bacteria and modulating IL-1β and TNF-α production. The use of Nal-P-113 in vivo might serve as a beneficial preventive or therapeutic approach for periodontitis. |
|
| dc.description |
https://deepblue.lib.umich.edu/bitstream/2027.42/138096/1/12906_2017_Article_1931.pdf |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.rights |
The Author(s). |
|
| dc.title |
Preventive effects of the novel antimicrobial peptide Nal-P-113 in a rat Periodontitis model by limiting the growth of Porphyromonas gingivalis and modulating IL-1β and TNF-α production |
|
| dc.type |
Article |
|