| dc.contributor |
Pharmacy, College of |
|
| dc.contributor |
Statistics, Department of |
|
| dc.creator |
Rosania, Gustavo R. |
|
| dc.creator |
Shedden, Kerby A. |
|
| dc.date |
2008-06-17T14:39:35Z |
|
| dc.date |
2008-06-17T14:39:35Z |
|
| dc.date |
2008 |
|
| dc.date.accessioned |
2022-05-19T13:30:54Z |
|
| dc.date.available |
2022-05-19T13:30:54Z |
|
| dc.identifier |
https://hdl.handle.net/2027.42/58874 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/117400 |
|
| dc.description |
This is part of a larger collection of archives of 1344 styryl compounds containing digital microscopic images of HeLa cells incubated with styryl compounds, along with other information about those compounds. ** The compounds were synthesized in a combinatorial synthesis involving 8 pyridinium/quinolinium groups (A-H) and 168 aldehyde groups (1-168). The compounds are identified by their A-H letter followed by their 1-168 number.
** Each of the 12 images in this archive is a 512x512 pixel image stored as a bzip2 compressed sequence of 2 byte unsigned short integers (little endian format), in standard raster order. ** Fluorescence in the Hoechst channel derives from Hoechst dye. Fluorescence in the other channels is presumed to derive from the styryl molecules, although cellular autofluorescence is also present. ** The images were acquired at 200X magnification with a 20X objective. The images were acquired with a 12 bit CCD camera so the greyscale intensities range from 0 to 4095. ** The "withdye" images were acquired after around 1 hour of incubation with the styryl probe. Next the probe was washed out using an onboard robotic pipetting system, and the "washout" images were acquired. ** The Hoechst dye images were acquired at 50msec exposure time, the other channels were acquired at both a 1sec and 200msec exposure time (Cy5 was only acquired at 1sec). ** The images were obtained using a Cellomics KineticScan instrument. Cells were kept alive and healthy in an onboard environmental control chamber. ** Styryl compounds were present at 100 micromolar concentration. |
|
| dc.description |
http://deepblue.lib.umich.edu/bitstream/2027.42/58874/1/A99.tar |
|
| dc.format |
The archive is in standard tar format and can be extracted using GNU tar or other software conforming to the tar standard. The files within the tar archive (except for the png image) are bzip2 compressed. |
|
| dc.format |
Each image is a 512x512 pixel image stored as a bzip2 compressed sequence of 2 byte unsigned short integers (little endian format), in standard raster order |
|
| dc.format |
2580480 bytes |
|
| dc.format |
1297 bytes |
|
| dc.format |
application/octet-stream |
|
| dc.format |
text/plain |
|
| dc.format |
application/octet-stream |
|
| dc.language |
en_US |
|
| dc.subject |
HeLa Cells |
|
| dc.subject |
Styryl Compound |
|
| dc.subject |
Hoechst Dye |
|
| dc.subject |
Pharmacy and Pharmacology |
|
| dc.subject |
Health Sciences |
|
| dc.title |
HeLa cells incubated with styryl compound A99 |
|
| dc.type |
Image |
|
| dc.type |
Dataset |
|