Green Bees : Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropism

Gusachenko, Olesya N.; Woodford, Luke; Balbirnie-Cumming, Katharin; Campbell, Ewan M.; Christie, Craig R.; Bowman, Alan S.; Evans, David J.

dc.contributor	University of Aberdeen.Medical Sciences
dc.contributor	University of Aberdeen.Biological Sciences
dc.contributor	University of Aberdeen.Biological Sciences (Research Theme)
dc.contributor	University of Aberdeen.Marine Alliance for Science and Technology for Scotland (MASTS)
dc.contributor	University of Aberdeen.Institute of Medical Sciences
dc.creator	Gusachenko, Olesya N.
dc.creator	Woodford, Luke
dc.creator	Balbirnie-Cumming, Katharin
dc.creator	Campbell, Ewan M.
dc.creator	Christie, Craig R.
dc.creator	Bowman, Alan S.
dc.creator	Evans, David J.
dc.date	2020-05-12T13:50:01Z
dc.date	2020-05-12T13:50:01Z
dc.date	2020-05
dc.date.accessioned	2022-05-24T06:10:19Z
dc.date.available	2022-05-24T06:10:19Z
dc.identifier	Gusachenko , O N , Woodford , L , Balbirnie-Cumming , K , Campbell , E M , Christie , C R , Bowman , A S & Evans , D J 2020 , ' Green Bees : Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropism ' , Viruses , vol. 12 , no. 5 , 532 . https://doi.org/10.3390/v12050532
dc.identifier	1999-4915
dc.identifier	PURE: 162968695
dc.identifier	PURE UUID: 3cceec8b-e7df-471f-a7a9-c80b86b19bea
dc.identifier	PubMed: 32408550
dc.identifier	Scopus: 85084786541
dc.identifier	https://hdl.handle.net/2164/14309
dc.identifier	https://doi.org/10.3390/v12050532
dc.identifier	http://www.scopus.com/inward/record.url?scp=85084786541&partnerID=8YFLogxK
dc.identifier	http://www.mdpi.com/1999-4915/12/5/532/s1
dc.identifier	12
dc.identifier	5
dc.identifier.uri	http://localhost:8080/xmlui/handle/CUHPOERS/117899
dc.description	Funding: This work was supported by grant funding from Biotechnology and Biological Sciences Research Council (BBSRC): BBSRC BB/M00337X/2 and BB/I000828/1. C.R.C. was supported by a KTN BBSRC CASE studentship BB/M503526/1 (http://www.bbsrc.ac.uk). C.R.C was part-funded by the Scottish Beekeeping Association (https://www.scottishbeekeepers.org.uk/) and the Animal Health and Welfare program by the Scottish Government. E.M.C. was supported by the Veterinary Medicines Directorate, Department for Environment Food & Rural Affairs (Project # VM0517) (https://www.gov.uk/government/organisations/veterinary-medicines-directorate). Acknowledgments: We would like to express our gratitude to Robert J Paxton (Martin Luther University Halle-Wittenberg) for providing a reference sequence for the construction of DWV-B RG system, Marcus Bischoff and Gill McVee (University of St Andrews) for helping with microscopic imaging set up, Javier Tello (University of St Andrews) for help and advice on sample cryosectioning, Ivan Gusachenko (University of St Andrews) for obtaining a photo of EGFP-expressing pupa, Ashley Pearson (University of St Andrews) for assistance in molecular biology assays, and Kirsten Bentley (University of St Andrews) for the high practical and moral support throughout the project.
dc.description	Peer reviewed
dc.description	Publisher PDF
dc.format	17
dc.format	application/pdf
dc.language	eng
dc.relation	Viruses
dc.rights	© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.subject	SDG 3 - Good Health and Well-being
dc.subject	insect viruses
dc.subject	honey bee
dc.subject	pollination
dc.subject	virus vector
dc.subject	Varroa
dc.subject	RNA viruses
dc.subject	DWV
dc.subject	reverse genetics
dc.subject	Reverse genetics
dc.subject	Pollination
dc.subject	Insect viruses
dc.subject	Virus vector
dc.subject	Honey bee
dc.subject	QH301 Biology
dc.subject	Infectious Diseases
dc.subject	Virology
dc.subject	Biotechnology and Biological Sciences Research Council (BBSRC)
dc.subject	BB/M00337X/2
dc.subject	BB/I000828/1
dc.subject	BB/M503526/1
dc.subject	Supplementary Data
dc.subject	QH301
dc.title	Green Bees : Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropism
dc.type	Journal article