Sangam: A Confluence of Knowledge Streams

Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids

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dc.creator Urquhart, Paula
dc.creator Parkin, Susan M.
dc.creator Nicolaou, Anna
dc.date 2009-12-07T16:13:04Z
dc.date 2009-12-07T16:13:04Z
dc.date 2009-12-07T16:13:04Z
dc.identifier Urquhart, P., Parkin, S. M. and Nicolaou, A. (2001). Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids. Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 65, No. 1, pp. 15-22.
dc.identifier http://hdl.handle.net/10454/4038
dc.description no
dc.description Human saphenous vein endothelial cells (HSVECs) derived from primary cultures of adult human veins constitute an excellent in vitro model for studying human endothelial metabolism. In this study we report the14C-labelled prostanoid profile of HSVECs under resting and stimulated conditions and the effect of the n-3 polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid on them. Results indicate that HSVECs while under resting conditions produce mainly prostaglandin F2 ¿(PGF2 ¿). After stimulation with calcium ionophore A23187, the cells were found to synthesise PGI2, PGE2and PGF2¿as major products and thromboxane B2and PGD2as minor products. Production of14C-labelled hydroxyeicosatetraenoic acids was not detected. Eicosapentaenoic acid was found to inhibit basal and stimulated prostanoid production whereas docosahexaenoic acid inhibited basal but strongly increased stimulated prostanoid production. These results may offer the basis for further studies aiming to investigate targets for pharmacological intervention in inflammatory conditions.
dc.language en
dc.publisher Harcourt
dc.relation http://dx.doi.org/10.1054/plef.2001.0282
dc.subject Human saphenous vein
dc.subject Endothelial cells
dc.subject Endothelial metabolism
dc.subject N-3 polyunsaturated fatty acids
dc.subject Dietary fatty acids
dc.subject Eicosapentaenoic acid
dc.subject Docosahexaenoic acid
dc.subject Prostaglandin F2 ¿(PGF2 ¿).
dc.subject Thromboxane
dc.subject Calcium
dc.title Profile of eicosanoids produced by human saphenous vein endothelial cells and the effect of dietary fatty acids
dc.type Article
dc.type published version paper


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