Sangam: A Confluence of Knowledge Streams

Fragmentation of N-linked glycans with a matrix-assisted laser desorption/ionization ion trap time-of-flight mass spectrometer.

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dc.creator Harvey, D.J.
dc.creator Martin, R.L.
dc.creator Jackson, K.A.
dc.creator Sutton, Chris W.
dc.date 2009-12-08T10:06:47Z
dc.date 2009-12-08T10:06:47Z
dc.date 2004
dc.identifier Harvey, D.J., Martin R.L., Jackson, K.A., Sutton, C.W. (2004). Rapid Commununications in Mass Spectrometry. Vol. 18, No. 24, pp. 2997-3007.
dc.identifier http://hdl.handle.net/10454/4039
dc.description no
dc.description N-Linked glycans were ionized from several matrices with a Shimadzu-Biotech AXIMA-QIT matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometer. [M+Na]+ ions were produced from all matrices and were accompanied by varying amounts of in-source fragmentation products. The least fragmentation was produced by 2,5-dihydroxybenzoic acid and the most by -cyano-4-hydroxycinnamic acid and 6-aza-2-thiothymine. Sialic acid loss was extensive but could be prevented by formation of methyl esters. Fragmentation produced typical low-energy-type spectra dominated by ions formed by glycosidic cleavages. MSn spectra (n = 3 and 4) were used to probe the pathways leading to the major diagnostic ions. Thus, for example, an ion that was formed by loss of the core GlcNAc residues and the 3-antenna was confirmed as being formed by a B/Y rather than a C/Z mechanism. The proposed structures of several cross-ring cleavage ions were confirmed and it was shown that MS3 spectra could be obtained from as little as 10 fmol of glycan
dc.language en
dc.publisher Wiley
dc.relation http://dx.doi.org/10.1002/rcm.1709
dc.subject N-linked glycans
dc.subject Matrix-assisted laser desorption
dc.subject MS3 spectra
dc.subject Ion trap
dc.title Fragmentation of N-linked glycans with a matrix-assisted laser desorption/ionization ion trap time-of-flight mass spectrometer.
dc.type Article
dc.type published version paper


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