| dc.creator |
Singh, J.C.I. |
|
| dc.creator |
Cruickshank, S.M. |
|
| dc.creator |
Newton, D.J. |
|
| dc.creator |
Wakenshaw, L. |
|
| dc.creator |
Graham, Anne M. |
|
| dc.creator |
Lan, J. |
|
| dc.creator |
Lodge, J.P.A. |
|
| dc.creator |
Felsburg, P.J. |
|
| dc.creator |
Carding, S.R. |
|
| dc.date |
2009-12-10T16:35:13Z |
|
| dc.date |
2009-12-10T16:35:13Z |
|
| dc.date |
2004 |
|
| dc.date |
2005 |
|
| dc.identifier |
Singh, J.C.I., Cruickshank, S.M., Newton, D.J. et al. (2004). Toll-like receptor-mediated responses of primary intestinal epithelial cells during the development of colitis. American Journal of Physiology Gastrointestinal and Liver Physiology. Vol. 288, No. 3, G514-G524. |
|
| dc.identifier |
http://hdl.handle.net/10454/4048 |
|
| dc.description |
no |
|
| dc.description |
The interleukin-2-deficient (IL-2¿/¿) mouse model of ulcerative colitis was used to test the hypothesis that colonic epithelial cells (CEC) directly respond to bacterial antigens and that alterations in Toll-like receptor (TLR)-mediated signaling may occur during the development of colitis. TLR expression and activation of TLR-mediated signaling pathways in primary CEC of healthy animals was compared with CEC in IL-2¿/¿ mice during the development of colitis. In healthy animals, CEC expressed functional TLR, and in response to the TLR4 ligand LPS, proliferated and secreted the cytokines IL-6 and monocyte chemoattractant protein-1 (MCP-1). However, the TLR-responsiveness of CEC in IL-2¿/¿ mice was different with decreased TLR4 responsiveness and augmented TLR2 responses that result in IL-6 and MCP-1 secretion. TLR signaling in CEC did not involve NF-B (p65) activation with the inhibitory p50 form of NF-B predominating in CEC in both the healthy and inflamed colon. Development of colitis was, however, associated with the activation of MAPK family members and upregulation of MyD88-independent signaling pathways characterized by increased caspase-1 activity and IL-18 production. These findings identify changes in TLR expression and signaling during the development of colitis that may contribute to changes in the host response to bacterial antigens seen in colitis. |
|
| dc.language |
en |
|
| dc.publisher |
The American Physiological Society |
|
| dc.relation |
http://dx.doi.org/10.1152/ajpgi.00377.2004 |
|
| dc.subject |
Ulcerative colitis |
|
| dc.subject |
Colon |
|
| dc.subject |
Interleukin-2-deficient (IL-2¿/¿) mouse model |
|
| dc.subject |
Colonic epithelial cells (CEC) |
|
| dc.subject |
Toll-like receptor (TLR)-mediated signaling |
|
| dc.title |
Toll-like receptor-mediated responses of primary intestinal epithelial cells during the development of colitis |
|
| dc.type |
Article |
|
| dc.type |
No full-text available in the repository |
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