Sangam: A Confluence of Knowledge Streams

High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy

Show simple item record

dc.creator Han, Yimo
dc.creator Fan, Xiao
dc.creator Wang, Haozhe
dc.creator Zhao, Fang
dc.creator Tully, Christopher G.
dc.creator Kong, Jing
dc.creator Yao, Nan
dc.creator Yan, Nieng
dc.date 2022-07-18T13:10:17Z
dc.date 2021-09-20T18:21:35Z
dc.date 2022-07-18T13:10:17Z
dc.date 2019-12
dc.date 2019-11
dc.date 2021-01-08T16:43:20Z
dc.date.accessioned 2023-02-17T19:59:51Z
dc.date.available 2023-02-17T19:59:51Z
dc.identifier 0027-8424
dc.identifier 1091-6490
dc.identifier https://hdl.handle.net/1721.1/132269.2
dc.identifier.uri http://localhost:8080/xmlui/handle/CUHPOERS/242078
dc.description © 2020 National Academy of Sciences. All rights reserved. Cryogenic electron microscopy (cryo-EM) has become one of the most powerful techniques to reveal the atomic structures and working mechanisms of biological macromolecules. New designs of the cryo-EM grids - aimed at preserving thin, uniform vitrified ice and improving protein adsorption - have been considered a promising approach to achieving higher resolution with the minimal amount of materials and data. Here, we describe a method for preparing graphene cryo-EM grids with up to 99% monolayer graphene coverage that allows for more than 70% grid squares for effective data acquisition with improved image quality and protein density. Using our graphene grids, we have achieved 2.6-Å resolution for streptavidin, with a molecular weight of 52 kDa, from 11, 000 particles. Our graphene grids increase the density of examined soluble, membrane, and lipoproteins by at least 5-fold, affording the opportunity for structural investigation of challenging proteins which cannot be produced in large quantity. In addition, our method employs only simple tools that most structural biology laboratories can access. Moreover, this approach supports customized grid designs targeting specific proteins, owing to its broad compatibility with a variety of nanomaterials.
dc.format application/octet-stream
dc.language en
dc.publisher National Academy of Sciences
dc.relation http://dx.doi.org/10.1073/pnas.1919114117
dc.relation Proceedings of the National Academy of Sciences
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.source PNAS
dc.title High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy
dc.type Article
dc.type http://purl.org/eprint/type/JournalArticle


Files in this item

Files Size Format View
1009.full.pdf 1.788Mb application/octet-stream View/Open

This item appears in the following Collection(s)

  • DSpace@MIT [2699]
    DSpace@MIT is a digital repository for MIT's research, including peer-reviewed articles, technical reports, working papers, theses, and more.

Show simple item record

Search DSpace


Advanced Search

Browse