Data for Gordon et al (2016) PLoS One - "A fine balance of synaptophysin levels underlies efficient retrieval of synaptobrevin II to synaptic vesicles". * Figure 1 – TIFFs displaying fluorescent images after immunostaining for either synaptophysin or synaptobrevin II in hippocampal neurones. A single experiment within that condition is described using the following format (2syb-pHl 2syp number 3 sybII Ab). * See also Figures 2 and 3 (https://doi.org/10.7488/ds/1311) – TIFF stacks displaying the synaptobrevin II-pHluorin response in hippocampal neurones to challenge with acid and alkali solutions. Each individual experiment is denoted with the same system (e.g. 20153025 CS07 mCer-Syp 1 + sybIIpH 1). * See also Figure 4 (https://doi.org/10.7488/ds/1312) - TIFF stacks displaying the synaptobrevin II-pHluorin response in hippocampal neurones to challenge with a train of 300 action potentials. Each individual experiment is denoted with the same system (e.g. 1sybII-pHl + 1mCer number 3). ## Note ## Image J or FIJI (both freeware) can be used to open the .tif files (http://imagej.nih.gov/ij/).