Sangam: A Confluence of Knowledge Streams

Alkyne-tagged PLGA allows direct visualisation of nanoparticles in vitro and ex vivo by stimulated Raman scattering microscopy

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dc.contributor BBSRC - Biotechnology and Biological Sciences Research Council
dc.contributor CRUK - Cancer Research UK
dc.contributor EPSRC - Engineering and Physical Sciences Research Council
dc.contributor Hulme, Alison
dc.creator Vanden-Hehir, Sally
dc.creator Cairns, Stefan
dc.creator Lee, Martin
dc.creator Zoupi, Lida
dc.creator Shaver, Michael
dc.creator Brunton, Valerie
dc.creator Williams, Anna
dc.creator Hulme, Alison
dc.date 2019-08-20T11:33:23Z
dc.date 2019-08-20T11:33:23Z
dc.date.accessioned 2023-02-17T20:52:59Z
dc.date.available 2023-02-17T20:52:59Z
dc.identifier Vanden-Hehir, Sally; Cairns, Stefan; Lee, Martin; Zoupi, Lida; Shaver, Michael; Brunton, Valerie; Williams, Anna; Hulme, Alison. (2019). Alkyne-tagged PLGA allows direct visualisation of nanoparticles in vitro and ex vivo by stimulated Raman scattering microscopy, [dataset]. University of Edinburgh. School of Chemistry. Hulme Research Group. https://doi.org/10.7488/ds/2608.
dc.identifier https://hdl.handle.net/10283/3399
dc.identifier https://doi.org/10.7488/ds/2608
dc.identifier.uri http://localhost:8080/xmlui/handle/CUHPOERS/244069
dc.description Polymeric nanoparticles (NPs) are attractive candidates for the controlled and targeted delivery of therapeutics in vitro and in vivo. However, detailed understanding of the uptake, location and ultimate cellular fate of the NPs is necessary in order to satisfy safety concerns, which is difficult because of the nanoscale size of these carriers. In this work, we show how small chemical labels can be appended to poly(lactic acid-co-glycolic acid) (PLGA) in order to synthesise NPs that can then be imaged by stimulated Raman scattering (SRS) microscopy, a vibrational imaging technique which can elucidate bond specific information in biological environments, such as the identification of alkyne signatures in modified PLGA terpolymers. We show that both deuterium and alkyne labelled NPs can be imaged within primary rat microglia, and the alkyne NPs can also be imaged ex vivo cortical mouse brain tissue. Immunohistochemical analysis confirms that the NPs localize in microglia in the mouse brain tissue, demonstrating that these NPs have the potential to deliver therapeutics selectively to microglia.
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dc.language eng
dc.publisher University of Edinburgh. School of Chemistry. Hulme Research Group
dc.relation https://pubs.acs.org/doi/10.1021/acs.biomac.9b01092
dc.rights Creative Commons Attribution 4.0 International Public License
dc.subject PLGA
dc.subject nanoparticles
dc.subject microglia
dc.subject Raman microscopy
dc.subject drug delivery
dc.subject stimulated Raman scattering
dc.subject Physical Sciences::Polymer Chemistry
dc.title Alkyne-tagged PLGA allows direct visualisation of nanoparticles in vitro and ex vivo by stimulated Raman scattering microscopy
dc.type dataset


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