| dc.contributor |
EPSRC - Engineering and Physical Sciences Research Council |
|
| dc.contributor |
Jepson, Alys |
|
| dc.creator |
Jepson, Alys |
|
| dc.creator |
Schwarz-Linek, Jana |
|
| dc.creator |
Ryan, Lloyd |
|
| dc.creator |
Ryadnov, Maxim |
|
| dc.creator |
Poon, Wilson CK |
|
| dc.date |
2016-01-14T19:09:23Z |
|
| dc.date |
2016-01-14T19:09:23Z |
|
| dc.date.accessioned |
2023-02-17T20:52:59Z |
|
| dc.date.available |
2023-02-17T20:52:59Z |
|
| dc.identifier |
Jepson, Alys; Schwarz-Linek, Jana; Ryan, Lloyd; Ryadnov, Maxim; Poon, Wilson CK. (2016). What is the ‘minimum inhibitory concentration’ (MIC) of pexiganan acting on Escherichia coli? - A cautionary case study, [dataset]. University of Edinburgh. https://doi.org/10.7488/ds/1309. |
|
| dc.identifier |
https://hdl.handle.net/10283/1885 |
|
| dc.identifier |
https://doi.org/10.7488/ds/1309 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/244070 |
|
| dc.description |
We measured the minimum inhibitory concentration (MIC) of the antimicrobial peptide pexiganan acting on Escherichia coli, and report an intrinsic variability in such measurements. These results led to a detailed study of the effect of pexiganan on the growth curve of E. coli, using a plate reader and manual plating (i.e. time-kill curves). The measured growth curves, together with single-cell observations and peptide depletion assays, suggested that addition of a sub-MIC concentration of pexiganan to a population of this bacterium killed a fraction of the cells, reducing peptide activity during the process, while leaving the remaining cells unaffected. This pharmacodynamic hypothesis suggests a considerable inoculum effect, which we quantified. Our results cast doubt on the use of the MIC as 'a measure of the concentration needed for peptide action' and show how 'coarse-grained' studies at the population level give vital information for the correct planning and interpretation of MIC measurements. |
|
| dc.description |
The .tif files constitute an animation which can be viewed using imagej, which is freely available. |
|
| dc.format |
text/plain |
|
| dc.format |
text/plain |
|
| dc.format |
text/plain |
|
| dc.format |
text/plain |
|
| dc.format |
text/plain |
|
| dc.format |
text/plain |
|
| dc.format |
text/plain |
|
| dc.format |
text/plain |
|
| dc.format |
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.format |
image/tiff |
|
| dc.language |
eng |
|
| dc.publisher |
University of Edinburgh |
|
| dc.relation |
https://doi.org/10.1007/978-3-319-32189-9_4 |
|
| dc.relation |
Jepson A.K., Schwarz-Linek J., Ryan L., Ryadnov M.G., Poon W.C.K. (2016) What Is the ‘Minimum Inhibitory Concentration’ (MIC) of Pexiganan Acting on Escherichia coli?—A Cautionary Case Study. In: Leake M. (eds) Biophysics of Infection. Advances in Experimental Medicine and Biology, vol 915. Springer, Cham |
|
| dc.rights |
Creative Commons Attribution 4.0 International Public License |
|
| dc.title |
What is the ‘minimum inhibitory concentration’ (MIC) of pexiganan acting on Escherichia coli? - A cautionary case study |
|
| dc.type |
dataset |
|