Sangam: A Confluence of Knowledge Streams

Distributing tasks via multiple input pathways increases cellular survival in stress

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dc.contributor BBSRC - Biotechnology and Biological Sciences Research Council
dc.contributor EPSRC - Engineering and Physical Sciences Research Council
dc.contributor Wellcome Trust
dc.contributor MRC - Medical Research Council
dc.contributor HFSP - Human Frontier Science Program
dc.contributor CONACyt
dc.contributor Swain, Peter
dc.creator Granados, Alejandro
dc.date 2017-05-05T09:58:07Z
dc.date 2017-05-05T09:58:07Z
dc.date.accessioned 2023-02-17T20:53:19Z
dc.date.available 2023-02-17T20:53:19Z
dc.identifier Granados, Alejandro. (2017). Distributing tasks via multiple input pathways increases cellular survival in stress, 2014-2015 [dataset]. University of Edinburgh. https://doi.org/10.7488/ds/2043.
dc.identifier https://hdl.handle.net/10283/2708
dc.identifier https://doi.org/10.7488/ds/2043
dc.identifier.uri http://localhost:8080/xmlui/handle/CUHPOERS/244101
dc.description Data from single-cell microscopy experiments for "Distributing tasks via multiple input pathways increases cellular survival in stress" by Granados, Crane, Montano-Gutierrez, Tanaka, Voliotis, & Swain. Improving in one aspect of a task can undermine performance in another, but how such opposing demands play out in single cells and impact on fitness is mostly unknown. Here we study budding yeast in dynamic environments of hyperosmotic stress and show how the corresponding signalling network increases cellular survival both by assigning the requirements of high response speed and high response accuracy to two separate input pathways and by having these pathways interact to converge on Hog1, a p38 MAP kinase. Cells with only the less accurate, reflex-like pathway are fitter in sudden stress, whereas cells with only the slow, more accurate pathway are fitter in fluctuating but increasing stress. Our results demonstrate that cellular signalling is vulnerable to trade-offs in performance, but that these trade-offs can be mitigated by assigning the opposing tasks to different signalling subnetworks. Such division of labour could function broadly within cellular signal transduction.
dc.description Text files of single-cell data in six ramps and six steps of osmotic stress for wild-type and STE11 and SSK1 deletion mutants of budding yeast. See README.txt.
dc.format text/plain
dc.format application/zip
dc.language eng
dc.publisher University of Edinburgh. School of Biological Sciences
dc.relation https://doi.org/10.7554/eLife.21415
dc.relation Granados, A. A., Crane, M., Montaño-gutierrez, L., Tanaka, R. J., Voliotis, M., & Swain, P. (2017). Distributing tasks via multiple input pathways increases cellular survival in stress. eLIFE, 6, 1-21. https://doi.org/10.7554/eLife.21415
dc.rights Creative Commons Attribution 4.0 International Public License
dc.subject Osmotic stress
dc.subject Budding yeast
dc.subject MAP kinase
dc.subject Hog1
dc.subject Nuclear translocation
dc.subject Volume
dc.subject Single cell
dc.subject Microfluidics
dc.subject Biological Sciences
dc.title Distributing tasks via multiple input pathways increases cellular survival in stress
dc.type dataset


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README.txt 1.813Kb text/plain View/Open

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