| dc.contributor | BBSRC - Biotechnology and Biological Sciences Research Council | |
| dc.contributor | Leverhulme Trust | |
| dc.contributor | Darwin Trust of Edinburgh | |
| dc.contributor | UKRI - UK Research and Innovation | |
| dc.contributor | Microsoft Research | |
| dc.contributor | Ho, Trevor Y. H. | |
| dc.creator | Ho, Trevor Y. H. | |
| dc.creator | Wang, Baojun | |
| dc.date | 2021-03-16T11:05:50Z | |
| dc.date | 2021-03-16T11:05:50Z | |
| dc.date.accessioned | 2023-02-17T20:54:18Z | |
| dc.date.available | 2023-02-17T20:54:18Z | |
| dc.identifier | Ho, Trevor Y. H.; Wang, Baojun. (2021). Source data and scripts for "A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction", [dataset]. University of Edinburgh. School of Biological Sciences. Centre for Synthetic and Systems Biology. https://doi.org/10.7488/ds/3001. | |
| dc.identifier | https://hdl.handle.net/10283/3857 | |
| dc.identifier | https://doi.org/10.7488/ds/3001 | |
| dc.identifier.uri | http://localhost:8080/xmlui/handle/CUHPOERS/244211 | |
| dc.description | Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augment a mini-Mu transposon-based screening approach and devise the intein-assisted bisection mapping (IBM) method. IBM robustly reveals clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further show that the use of inteins expands functional sequence space for splitting a protein. We also demonstrate the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins, and that basal activities of highly active proteins can be mitigated by splitting them. Our work offers a generalizable and systematic route towards creating split protein-intein fusions for synthetic biology. | |
| dc.description | Secondary structures of mCherry, TEM-1 beta-lactamase and TetR are obtained from Protein Data Bank, with accession codes of PDB: 2H5Q, PDB: 1ZG4, and PDB: 4AC0, respectively | |
| dc.format | text/html | |
| dc.format | application/x-ipynb+json | |
| dc.format | text/html | |
| dc.format | application/x-ipynb+json | |
| dc.format | text/html | |
| dc.format | application/octet-stream | |
| dc.format | application/x-ipynb+json | |
| dc.format | application/pdf | |
| dc.format | text/csv | |
| dc.format | text/html | |
| dc.format | application/x-ipynb+json | |
| dc.format | text/html | |
| dc.format | application/x-ipynb+json | |
| dc.format | text/html | |
| dc.format | application/x-ipynb+json | |
| dc.format | application/zip | |
| dc.language | eng | |
| dc.publisher | University of Edinburgh. School of Biological Sciences. Centre for Synthetic and Systems Biology | |
| dc.relation | https://doi.org/10.1101/2020.11.30.381921 | |
| dc.relation | https://doi.org/10.7488/ds/2954 | |
| dc.rights | Creative Commons Attribution 4.0 International Public License | |
| dc.source | Protein Data Bank | |
| dc.subject | split protein | |
| dc.subject | split intein | |
| dc.subject | bisection mapping | |
| dc.subject | logic gates | |
| dc.subject | inducibility | |
| dc.subject | post-translational control | |
| dc.subject | Biological Sciences::Biotechnology | |
| dc.title | Source data and scripts for "A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction" | |
| dc.type | dataset | |
| dc.coverage | UK | |
| dc.coverage | UNITED KINGDOM |