Sangam: A Confluence of Knowledge Streams

The Effect Ultrasound and Surfactants on Nanobubbles Efficacy against Listeria innocua and Escherichia coli O157:H7, in Cell Suspension and on Fresh Produce Surfaces

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dc.contributor Virginia Seafood Agricultural Research and Extension Center
dc.contributor Food Science and Technology
dc.creator Rafeeq, Shamil
dc.creator Ovissipour, Mahmoudreza
dc.date 2021-09-27T12:24:06Z
dc.date 2021-09-27T12:24:06Z
dc.date 2021-09-12
dc.date 2021-09-25T23:32:55Z
dc.date.accessioned 2023-02-28T17:40:27Z
dc.date.available 2023-02-28T17:40:27Z
dc.identifier Rafeeq, S.; Ovissipour, R. The Effect Ultrasound and Surfactants on Nanobubbles Efficacy against Listeria innocua and Escherichia coli O157:H7, in Cell Suspension and on Fresh Produce Surfaces. Foods 2021, 10, 2154.
dc.identifier http://hdl.handle.net/10919/105065
dc.identifier https://doi.org/10.3390/foods10092154
dc.identifier.uri http://localhost:8080/xmlui/handle/CUHPOERS/266614
dc.description Removing foodborne pathogens from food surfaces and inactivating them in wash water are critical steps for reducing the number of foodborne illnesses. In this study we evaluated the impact of surfactants on enhancing nanobubbles’ efficacy on <i>Escherichia coli</i> O157:H7, and <i>Listeria innocua</i> removal from spinach leaves. We evaluated the synergistic impact of nanobubbles and ultrasound on these two pathogens inactivation in the cell suspension. The results indicated that nanobubbles or ultrasound alone could not significantly reduce bacteria in cell suspension after 15 min. However, a combination of nanobubbles and ultrasonication caused more than 6 log cfu/mL reduction after 15 min, and 7 log cfu/mL reduction after 10 min of <i>L. innocua</i> and <i>E. coli</i>, respectively. Nanobubbles also enhanced bacterial removal from spinach surface in combination with ultrasonication. Nanobubbles with ultrasound removed more than 2 and 4 log cfu/cm<sup>2</sup> of <i>L. innocua</i> and <i>E. coli</i>, respectively, while ultrasound alone caused 0.5 and 1 log cfu/cm<sup>2</sup> of <i>L. innocua</i> and <i>E. coli</i> reduction, respectively. No reduction was observed in the solutions with PBS and nanobubbles. Adding food-grade surfactants (0.1% Sodium dodecyl sulfate-SDS, and 0.1% Tween 20), did not significantly enhance nanobubbles efficacy on bacterial removal from spinach surface.
dc.description Published version
dc.format application/pdf
dc.format application/pdf
dc.language en
dc.publisher MDPI
dc.rights Creative Commons Attribution 4.0 International
dc.rights http://creativecommons.org/licenses/by/4.0/
dc.title The Effect Ultrasound and Surfactants on Nanobubbles Efficacy against Listeria innocua and Escherichia coli O157:H7, in Cell Suspension and on Fresh Produce Surfaces
dc.title Foods
dc.type Article - Refereed
dc.type Text


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