| dc.contributor |
Agronomy |
|
| dc.creator |
Crosby, Kevin Edward |
|
| dc.date |
2017-05-24T18:19:04Z |
|
| dc.date |
2017-05-24T18:19:04Z |
|
| dc.date |
1982 |
|
| dc.date.accessioned |
2023-03-01T08:10:21Z |
|
| dc.date.available |
2023-03-01T08:10:21Z |
|
| dc.identifier |
http://hdl.handle.net/10919/77769 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/276606 |
|
| dc.description |
Soybean, Glycine max (L.) Merr., fruits 10-25 mm long contained nine fractions having cytokinin-like activity detectable by the Amaranthus bioassay. These fractions were derived from purification of extracts from 15 g of immature fruits. The fractions were purified by polyvinylpyrrolidone (PVP), paper, Sephadex LH-20 and high performance liquid chromatography. The fractions separated on the basis of polarity into polar, moderately polar and relatively non-polar groups, compared to zeatin. The polar fraction was the single most bioactive fraction and the active substance was tentatively identified as a zeatin glucoside or zeatin riboside glucoside. The moderately polar fraction contained bioactive substances that co-eluted with zeatin and zeatin riboside in four chromatographic procedures. The relatively non-polar fraction contained two bioactive components, one chromatographically resembling 2iPA, and the second less polar. A conjugated cytokinin was released from the original aqueous phase by alkaline phosphatase hydrolysis. This substance co-eluted with zeatin riboside on Sephadex LH-20. Further purification and analysis by multiple ion monitoring GC-MS did not yield conclusive identification of the cytokinins. Based on bioassay, the biological estimates of cytokinin-like substances present in the extracts were below the detection limit of the GC-MS. Obtaining sufficiently large samples of soybean fruits at the proper stage of physiological interest complicated definitive structural identification based on the purification and identification techniques used. |
|
| dc.description |
Ph. D. |
|
| dc.format |
ix, 91, [2] leaves |
|
| dc.format |
application/pdf |
|
| dc.format |
application/pdf |
|
| dc.language |
en_US |
|
| dc.publisher |
Virginia Polytechnic Institute and State University |
|
| dc.relation |
OCLC# 9247539 |
|
| dc.rights |
In Copyright |
|
| dc.rights |
http://rightsstatements.org/vocab/InC/1.0/ |
|
| dc.subject |
LD5655.V856 1982.C767 |
|
| dc.subject |
Soybean |
|
| dc.subject |
Cytokinins |
|
| dc.title |
Identification of soybean, Glycine max (L.) Merr., cytokinins |
|
| dc.type |
Dissertation |
|
| dc.type |
Text |
|