| dc.contributor |
Biochemistry and Nutrition |
|
| dc.contributor |
Bevan, David R. |
|
| dc.contributor |
Ebel, Richard E. |
|
| dc.contributor |
Sitz, Thomas O. |
|
| dc.contributor |
Potts, Malcolm |
|
| dc.contributor |
Veit, Hugo P. |
|
| dc.creator |
Ankrapp, David P. |
|
| dc.date |
2014-03-14T21:20:45Z |
|
| dc.date |
2014-03-14T21:20:45Z |
|
| dc.date |
1993-05-05 |
|
| dc.date |
2005-10-10 |
|
| dc.date |
2005-10-10 |
|
| dc.date |
2005-10-10 |
|
| dc.date.accessioned |
2023-03-01T08:11:17Z |
|
| dc.date.available |
2023-03-01T08:11:17Z |
|
| dc.identifier |
etd-10102005-131608 |
|
| dc.identifier |
http://hdl.handle.net/10919/39726 |
|
| dc.identifier |
http://scholar.lib.vt.edu/theses/available/etd-10102005-131608/ |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/276737 |
|
| dc.description |
The concentration of insulin-like growth factor I (IGF-I) in tissue taken from human non-small cell lung carcinoma (NSCLC) is 1.4- to 7-fold higher than the concentration of IGF-I in the surrounding normal lung tissue and therefore IGF-I may be involved in the growth of NSCLC. In this study it was determined that NSCLC cell lines (A549, A427, SK-LU-1) expressed the type I IGF-I receptor protein and IGF-I stimulated the proliferation of low density plated (2000 cells/cm² growth area) carcinoma cells by 1.6- to 3- fold above control after a four day inCUbation period under serum-free conditions (A549, A427) or in the presence of 0.25% serum (SK-LU-1). In addition, when added to detergent-solubilized type I IGF receptors from A549 cells, IGF-I stimulated [1] a dose-dependent increase in the autophosphorylation of the type I IGF receptor, and [2] a dose-dependent increase (1.5- to 4-fold) in the phosphorylation of a tyrosine kinase-specific substrate. These results suggest that the growth promoting activity of IGF-I for the lung carcinoma cells was mediated through the activation of the type I IGF receptor. |
|
| dc.description |
Ph. D. |
|
| dc.format |
xiii, 134 leaves |
|
| dc.format |
BTD |
|
| dc.format |
application/pdf |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.publisher |
Virginia Tech |
|
| dc.relation |
OCLC# 28732754 |
|
| dc.relation |
LD5655.V856_1993.A557.pdf |
|
| dc.rights |
In Copyright |
|
| dc.rights |
http://rightsstatements.org/vocab/InC/1.0/ |
|
| dc.subject |
LD5655.V856 1993.A557 |
|
| dc.subject |
Cancer cells -- Growth |
|
| dc.subject |
Lungs -- Cancer |
|
| dc.subject |
Somatomedin |
|
| dc.title |
The effect of insulin-like growth factor-I (IGF-I), and an IGF-I-like factor secreted by human lung fibroblasts, on the growth of human lung carcinoma cells in vitro |
|
| dc.type |
Dissertation |
|
| dc.type |
Text |
|