| dc.contributor |
Massachusetts Institute of Technology. Department of Chemistry |
|
| dc.contributor |
Lippard, Stephen J. |
|
| dc.contributor |
Tinberg, Christine E. |
|
| dc.contributor |
Tonzetich, Zachary J. |
|
| dc.contributor |
Do, Loi Hung |
|
| dc.contributor |
Lippard, Stephen J. |
|
| dc.creator |
Wang, Hongxin |
|
| dc.creator |
Yoda, Yoshitaka |
|
| dc.creator |
Cramer, Stephen P. |
|
| dc.creator |
Tinberg, Christine E. |
|
| dc.creator |
Tonzetich, Zachary J. |
|
| dc.creator |
Do, Loi Hung |
|
| dc.creator |
Lippard, Stephen J. |
|
| dc.date |
2011-12-15T18:34:14Z |
|
| dc.date |
2011-12-15T18:34:14Z |
|
| dc.date |
2010-12 |
|
| dc.date.accessioned |
2023-03-01T18:10:19Z |
|
| dc.date.available |
2023-03-01T18:10:19Z |
|
| dc.identifier |
0002-7863 |
|
| dc.identifier |
1520-5126 |
|
| dc.identifier |
http://hdl.handle.net/1721.1/67696 |
|
| dc.identifier |
Tinberg, Christine E. et al. “Characterization of Iron Dinitrosyl Species Formed in the Reaction of Nitric Oxide with a Biological Rieske Center.” Journal of the American Chemical Society 132 (2010): 18168-18176. Web. 15 Dec. 2011. © 2011 American Chemical Society |
|
| dc.identifier |
https://orcid.org/0000-0002-2693-4982 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/279019 |
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| dc.description |
Reactions of nitric oxide with cysteine-ligated iron−sulfur cluster proteins typically result in disassembly of the iron−sulfur core and formation of dinitrosyl iron complexes (DNICs). Here we report the first evidence that DNICs also form in the reaction of NO with Rieske-type [2Fe-2S] clusters. Upon treatment of a Rieske protein, component C of toluene/o-xylene monooxygenase from Pseudomonas sp. OX1, with an excess of NO(g) or NO-generators S-nitroso-N-acetyl-d,l-pencillamine and diethylamine NONOate, the absorbance bands of the [2Fe-2S] cluster are extinguished and replaced by a new feature that slowly grows in at 367 nm. Analysis of the reaction products by electron paramagnetic resonance, Mössbauer, and nuclear resonance vibrational spectroscopy reveals that the primary product of the reaction is a thiolate-bridged diiron tetranitrosyl species, [Fe[subscript 2](μ-SCys)[subscript 2](NO)[subscript 4]], having a Roussin’s red ester (RRE) formula, and that mononuclear DNICs account for only a minor fraction of nitrosylated iron. Reduction of this RRE reaction product with sodium dithionite produces the one-electron-reduced RRE, having absorptions at 640 and 960 nm. These results demonstrate that NO reacts readily with a Rieske center in a protein and suggest that dinuclear RRE species, not mononuclear DNICs, may be the primary iron dinitrosyl species responsible for the pathological and physiological effects of nitric oxide in such systems in biology. |
|
| dc.description |
National Institute of General Medical Sciences (U.S.) (grant GM032134) |
|
| dc.description |
United States. Dept. of Energy (DOE OBER) |
|
| dc.description |
National Institute of General Medical Sciences (U.S.) (GM065440) |
|
| dc.description |
National Institutes of Health (U.S.). Biotechnology Training Fellowship (Grant T32 GM08334) |
|
| dc.description |
National Institute of General Medical Sciences (U.S.) (1 F32 GM082031-03) |
|
| dc.format |
application/pdf |
|
| dc.language |
en_US |
|
| dc.publisher |
American Chemical Society |
|
| dc.relation |
http://dx.doi.org/10.1021/ja106290p |
|
| dc.relation |
Journal of the American Chemical Society |
|
| dc.rights |
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. |
|
| dc.source |
Prof. Lippard via Erja Kajosalo |
|
| dc.title |
Characterization of Iron Dinitrosyl Species Formed in the Reaction of Nitric Oxide with a Biological Rieske Center |
|
| dc.type |
Article |
|
| dc.type |
http://purl.org/eprint/type/JournalArticle |
|