| dc.contributor |
Harvard University--MIT Division of Health Sciences and Technology |
|
| dc.contributor |
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
|
| dc.contributor |
Massachusetts Institute of Technology. Research Laboratory of Electronics |
|
| dc.contributor |
Schubert, Christian R. |
|
| dc.contributor |
Stultz, Collin M. |
|
| dc.creator |
Liu, Judy S. |
|
| dc.creator |
Schubert, Christian R. |
|
| dc.creator |
Fu, Xiaoqin |
|
| dc.creator |
Fourniol, Franck J. |
|
| dc.creator |
Jaiswal, Jyoti K. |
|
| dc.creator |
Houdusse, Anne |
|
| dc.creator |
Stultz, Collin M. |
|
| dc.creator |
Moores, Carolyn A. |
|
| dc.creator |
Walsh, Christopher A. |
|
| dc.creator |
Schubert, Christian R. |
|
| dc.creator |
Stultz, Collin M. |
|
| dc.date |
2013-11-18T16:06:49Z |
|
| dc.date |
2013-11-18T16:06:49Z |
|
| dc.date |
2012-07 |
|
| dc.date |
2012-05 |
|
| dc.date.accessioned |
2023-03-01T18:10:52Z |
|
| dc.date.available |
2023-03-01T18:10:52Z |
|
| dc.identifier |
10972765 |
|
| dc.identifier |
http://hdl.handle.net/1721.1/82159 |
|
| dc.identifier |
Liu, Judy S., Christian R. Schubert, Xiaoqin Fu, Franck J. Fourniol, Jyoti K. Jaiswal, Anne Houdusse, Collin M. Stultz, Carolyn A. Moores, and Christopher A. Walsh. “Molecular Basis for Specific Regulation of Neuronal Kinesin-3 Motors by Doublecortin Family Proteins.” Molecular Cell 47, no. 5 (September 2012): 707-721. © 2012 Elsevier Inc. |
|
| dc.identifier |
https://orcid.org/0000-0002-3415-242X |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/279054 |
|
| dc.description |
Doublecortin (Dcx) defines a growing family of microtubule (MT)-associated proteins (MAPs) involved in neuronal migration and process outgrowth. We show that Dcx is essential for the function of Kif1a, a kinesin-3 motor protein that traffics synaptic vesicles. Neurons lacking Dcx and/or its structurally conserved paralogue, doublecortin-like kinase 1 (Dclk1), show impaired Kif1a-mediated transport of Vamp2, a cargo of Kif1a, with decreased run length. Human disease-associated mutations in Dcx's linker sequence (e.g., W146C, K174E) alter Kif1a/Vamp2 transport by disrupting Dcx/Kif1a interactions without affecting Dcx MT binding. Dcx specifically enhances binding of the ADP-bound Kif1a motor domain to MTs. Cryo-electron microscopy and subnanometer-resolution image reconstruction reveal the kinesin-dependent conformational variability of MT-bound Dcx and suggest a model for MAP-motor crosstalk on MTs. Alteration of kinesin run length by MAPs represents a previously undiscovered mode of control of kinesin transport and provides a mechanism for regulation of MT-based transport by local signals. |
|
| dc.description |
National Institutes of Health (U.S.) (Grant NIH-P30-HD-18655) |
|
| dc.description |
National Institutes of Health (U.S.) (Grant 2T32NS007473-11) |
|
| dc.description |
National Institutes of Health (U.S.) (Grant 2T32NS007484-11) |
|
| dc.description |
National Institutes of Health (U.S.) (Grant 1F32D070549-01) |
|
| dc.description |
National Science Foundation (U.S.) (CAREER Award) |
|
| dc.description |
National Institutes of Health (U.S.) (Grant 5R21NS063185-02) |
|
| dc.format |
application/pdf |
|
| dc.language |
en_US |
|
| dc.publisher |
Elsevier |
|
| dc.relation |
http://dx.doi.org/10.1016/j.molcel.2012.06.025 |
|
| dc.relation |
Molecular Cell |
|
| dc.rights |
Article is available under a Creative Commons license. |
|
| dc.rights |
http://creativecommons.org/ |
|
| dc.source |
PMC |
|
| dc.title |
Molecular Basis for Specific Regulation of Neuronal Kinesin-3 Motors by Doublecortin Family Proteins |
|
| dc.type |
Article |
|
| dc.type |
http://purl.org/eprint/type/JournalArticle |
|