| dc.contributor |
Massachusetts Institute of Technology. Department of Biology |
|
| dc.creator |
Franklin, Reuben |
|
| dc.creator |
Guo, Yiming |
|
| dc.creator |
He, Shiyang |
|
| dc.creator |
Chen, Meijuan |
|
| dc.creator |
Ji, Fei |
|
| dc.creator |
Zhou, Xinyue |
|
| dc.creator |
Frankhouser, David |
|
| dc.creator |
Do, Brian T |
|
| dc.creator |
Chiem, Carmen |
|
| dc.creator |
Jang, Mihyun |
|
| dc.creator |
Blanco, M Andres |
|
| dc.creator |
Vander Heiden, Matthew G |
|
| dc.creator |
Rockne, Russell C |
|
| dc.creator |
Ninova, Maria |
|
| dc.creator |
Sykes, David B |
|
| dc.creator |
Hochedlinger, Konrad |
|
| dc.creator |
Lu, Rui |
|
| dc.creator |
Sadreyev, Ruslan I |
|
| dc.creator |
Murn, Jernej |
|
| dc.creator |
Volk, Andrew |
|
| dc.creator |
Cheloufi, Sihem |
|
| dc.date |
2023-01-06T15:01:57Z |
|
| dc.date |
2023-01-06T15:01:57Z |
|
| dc.date |
2022 |
|
| dc.date |
2023-01-06T14:48:13Z |
|
| dc.date.accessioned |
2023-03-01T18:11:38Z |
|
| dc.date.available |
2023-03-01T18:11:38Z |
|
| dc.identifier |
https://hdl.handle.net/1721.1/146999 |
|
| dc.identifier |
Franklin, Reuben, Guo, Yiming, He, Shiyang, Chen, Meijuan, Ji, Fei et al. 2022. "Regulation of chromatin accessibility by the histone chaperone CAF-1 sustains lineage fidelity." Nature Communications, 13 (1). |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/279102 |
|
| dc.description |
<jats:title>Abstract</jats:title><jats:p>Cell fate commitment is driven by dynamic changes in chromatin architecture and activity of lineage-specific transcription factors (TFs). The chromatin assembly factor-1 (CAF-1) is a histone chaperone that regulates chromatin architecture by facilitating nucleosome assembly during DNA replication. Accumulating evidence supports a substantial role of CAF-1 in cell fate maintenance, but the mechanisms by which CAF-1 restricts lineage choice remain poorly understood. Here, we investigate how CAF-1 influences chromatin dynamics and TF activity during lineage differentiation. We show that CAF-1 suppression triggers rapid differentiation of myeloid stem and progenitor cells into a mixed lineage state. We find that CAF-1 sustains lineage fidelity by controlling chromatin accessibility at specific loci, and limiting the binding of ELF1 TF at newly-accessible diverging regulatory elements. Together, our findings decipher key traits of chromatin accessibility that sustain lineage integrity and point to a powerful strategy for dissecting transcriptional circuits central to cell fate commitment.</jats:p> |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.publisher |
Springer Science and Business Media LLC |
|
| dc.relation |
10.1038/S41467-022-29730-6 |
|
| dc.relation |
Nature Communications |
|
| dc.rights |
Creative Commons Attribution 4.0 International license |
|
| dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
|
| dc.source |
Nature |
|
| dc.title |
Regulation of chromatin accessibility by the histone chaperone CAF-1 sustains lineage fidelity |
|
| dc.type |
Article |
|
| dc.type |
http://purl.org/eprint/type/JournalArticle |
|