| dc.contributor |
Massachusetts Institute of Technology. Department of Biology |
|
| dc.contributor |
MIT Program in Writing & Humanistic Studies |
|
| dc.contributor |
Koch Institute for Integrative Cancer Research at MIT |
|
| dc.contributor |
Leung, Anthony K.L. |
|
| dc.contributor |
Vyas, Sejal Kamlesh |
|
| dc.contributor |
Rood, Jennifer Evelyn |
|
| dc.contributor |
Bhutkar, Arjun (AJ) |
|
| dc.contributor |
Sharp, Phillip A. |
|
| dc.contributor |
Chang, Paul |
|
| dc.creator |
Leung, Anthony |
|
| dc.creator |
Bhutkar, Arjun (AJ) |
|
| dc.creator |
Chang, Paul |
|
| dc.creator |
Vyas, Sejal Kamlesh |
|
| dc.creator |
Rood, Jennifer Evelyn |
|
| dc.creator |
Leung, Anthony |
|
| dc.creator |
Sharp, Phillip A. |
|
| dc.date |
2014-01-27T18:31:08Z |
|
| dc.date |
2014-01-27T18:31:08Z |
|
| dc.date |
2011-05 |
|
| dc.date |
2011-03 |
|
| dc.date.accessioned |
2023-03-01T18:12:09Z |
|
| dc.date.available |
2023-03-01T18:12:09Z |
|
| dc.identifier |
10972765 |
|
| dc.identifier |
1097-4164 |
|
| dc.identifier |
http://hdl.handle.net/1721.1/84591 |
|
| dc.identifier |
Leung, Anthony K.L., Sejal Vyas, Jennifer E. Rood, Arjun Bhutkar, Phillip A. Sharp, and Paul Chang. “Poly(ADP-Ribose) Regulates Stress Responses and MicroRNA Activity in the Cytoplasm.” Molecular Cell 42, no. 4 (May 2011): 489-499. Copyright © 2011 Elsevier Inc. |
|
| dc.identifier |
https://orcid.org/0000-0003-1465-1691 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/279137 |
|
| dc.description |
Poly(ADP-ribose) is a major regulatory macromolecule in the nucleus, where it regulates transcription, chromosome structure, and DNA damage repair. Functions in the interphase cytoplasm are less understood. Here, we identify a requirement for poly(ADP-ribose) in the assembly of cytoplasmic stress granules, which accumulate RNA-binding proteins that regulate the translation and stability of mRNAs upon stress. We show that poly(ADP-ribose), six specific poly(ADP-ribose) polymerases, and two poly(ADP-ribose) glycohydrolase isoforms are stress granule components. A subset of stress granule proteins, including microRNA-binding Argonaute family members Ago1–4, are modified by poly(ADP-ribose), and such modification increases upon stress, a condition when both microRNA-mediated translational repression and microRNA-directed mRNA cleavage are relieved. Similar relief of repression is also observed upon overexpression of specific poly(ADP-ribose) polymerases or, conversely, upon knockdown of glycohydrolase. We conclude that poly(ADP-ribose) is a key regulator of posttranscriptional gene expression in the cytoplasm. |
|
| dc.description |
National Institutes of Health (U.S.) (Grant RO1-CA133404) |
|
| dc.description |
National Cancer Institute (U.S.) (Grant PO1-CA42063) |
|
| dc.description |
National Cancer Institute (U.S.) (Cancer Center Support Core Grant P30-CA14051) |
|
| dc.format |
application/pdf |
|
| dc.language |
en_US |
|
| dc.publisher |
Elsevier |
|
| dc.relation |
http://dx.doi.org/10.1016/j.molcel.2011.04.015 |
|
| dc.relation |
Molecular Cell |
|
| dc.rights |
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. |
|
| dc.source |
Elsevier Open Archive |
|
| dc.title |
Poly(ADP-Ribose) Regulates Stress Responses and MicroRNA Activity in the Cytoplasm |
|
| dc.type |
Article |
|
| dc.type |
http://purl.org/eprint/type/JournalArticle |
|