| dc.contributor |
Biomedical Sciences and Pathobiology |
|
| dc.contributor |
Physics |
|
| dc.creator |
McCutcheon, Kelly |
|
| dc.creator |
Bandara, Aloka B. |
|
| dc.creator |
Zuo, Ziwei |
|
| dc.creator |
Heflin, James R. |
|
| dc.creator |
Inzana, Thomas J. |
|
| dc.date |
2019-05-31T11:52:05Z |
|
| dc.date |
2019-05-31T11:52:05Z |
|
| dc.date |
2019-05-21 |
|
| dc.date |
2019-05-31T06:01:59Z |
|
| dc.date.accessioned |
2023-03-01T18:52:04Z |
|
| dc.date.available |
2023-03-01T18:52:04Z |
|
| dc.identifier |
McCutcheon, K.; Bandara, A.B.; Zuo, Z.; Heflin, J.R.; Inzana, T.J. The Application of a Nanomaterial Optical Fiber Biosensor Assay for Identification of Brucella Nomenspecies. Biosensors 2019, 9, 64. |
|
| dc.identifier |
http://hdl.handle.net/10919/89653 |
|
| dc.identifier |
https://doi.org/10.3390/bios9020064 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/281580 |
|
| dc.description |
Bacteria in the genus <i>Brucella</i> are the cause of brucellosis in humans and many domestic and wild animals. A rapid and culture-free detection assay to detect <i>Brucella</i> in clinical samples would be highly valuable. Nanomaterial optical fiber biosensors (NOFS) are capable of recognizing DNA hybridization events or other analyte interactions with high specificity and sensitivity. Therefore, a NOFS assay was developed to detect <i>Brucella</i> DNA from cultures and in tissue samples from infected mice. An ionic self-assembled multilayer (ISAM) film was coupled to a long-period grating optical fiber, and a nucleotide probe complementary to the <i>Brucella</i> IS<i>711</i> region and modified with biotin was bound to the ISAM by covalent conjugation. When the ISAM/probe duplex was exposed to lysate containing ≥100 killed cells of <i>Brucella</i>, or liver or spleen tissue extracts from <i>Brucella-</i>infected mice, substantial attenuation of light transmission occurred, whereas exposure of the complexed fiber to non-<i>Brucella</i> gram-negative bacteria or control tissue samples resulted in negligible attenuation of light transmission. Oligonucleotide probes specific for <i>B. abortus</i>, <i>B. melitensis</i>, and <i>B. suis</i> could also be used to detect and differentiate these three nomenspecies. In summary, the NOFS biosensor assay detected three nomenspecies of <i>Brucella</i> without the use of polymerase chain reaction within 30 min and could specifically detect low numbers of this bacterium in clinical samples. |
|
| dc.description |
Published version |
|
| dc.format |
application/pdf |
|
| dc.format |
application/pdf |
|
| dc.language |
en |
|
| dc.publisher |
MDPI |
|
| dc.rights |
Creative Commons Attribution 4.0 International |
|
| dc.rights |
http://creativecommons.org/licenses/by/4.0/ |
|
| dc.subject |
Brucella abortus |
|
| dc.subject |
Brucella melitensis |
|
| dc.subject |
Brucella suis |
|
| dc.subject |
optical fiber |
|
| dc.subject |
biosensor |
|
| dc.subject |
nucleotide probe |
|
| dc.subject |
light transmission |
|
| dc.subject |
diagnosis |
|
| dc.title |
The Application of a Nanomaterial Optical Fiber Biosensor Assay for Identification of Brucella Nomenspecies |
|
| dc.title |
Biosensors |
|
| dc.type |
Article - Refereed |
|
| dc.type |
Text |
|