In this study, a comprehensive methodology was adapted to determine the environmental flow regime of “La Yerbabuena”, a temporary stream located in the Aguascalientes Valley, Mexico. The analysis was divided into four stages: the geomorphological watershed analysis, a hydrologic analysis, hydraulic modeling, and environmental analysis. The main geomorphological features of the study area were defined from maps in the spatial block, and with them, a synthetic series of daily and monthly discharge was determined and further used in the next stages. In the hydrological stage, the IHA (Indicators of Hydrologic Alteration) methodology and the procedures from the Mexican regulation, named NMX-159, were applied to the stream, and their results were comparatively analyzed. A similar interannual flow variation from both methodologies was found for wet and dry seasons, ranging from 0.010 to 0.108 m³/s. In the hydraulic modeling stage, a micro-basin part of the stream was modeled in the software HEC RAS, observing that the IHA methodology results had water levels that matched the baseflow of the stream, which allows understanding the hydraulic behavior of the water flow through the generation of different profiles in function of the rainy season. Finally, for the environmental stage, the hydrological health of the stream was evaluated using the software Flow Health, additionally observing that the IHA methodology was closer to the desired water level of the reference. This study demonstrates that the proposed methodology achieves the objectives defined by the NMX-159, which establishes a streamflow regime considering a natural interval of hydrologic variability in both ordinary and after-disturbance conditions. This application of the methodology for temporary streams provides an understanding of the hydrological behavior of the environmental flow throughout the year, and regarding the existing regulations, it presents a correlation with the obtained results, as well as greater precision in the dry season.Published version