Sangam: A Confluence of Knowledge Streams

The expression profile of Aedes albopictus miRNAs is altered by dengue virus serotype-2 infection

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dc.contributor Biochemistry
dc.creator Liu, Yanxia
dc.creator Zhou, Yanhe
dc.creator Wu, Jinya
dc.creator Zheng, Peiming
dc.creator Li, Yiji
dc.creator Zheng, Xiaoying
dc.creator Puthiyakunnon, Santhosh
dc.creator Tu, Zhijian Jake
dc.creator Chen, Xiaoguang
dc.date 2015-07-31T16:41:12Z
dc.date 2015-07-31T16:41:12Z
dc.date 2015-04-16
dc.date 2015-07-31T16:41:12Z
dc.date.accessioned 2023-03-01T18:54:44Z
dc.date.available 2023-03-01T18:54:44Z
dc.identifier Cell & Bioscience. 2015 Apr 16;5(1):16
dc.identifier http://hdl.handle.net/10919/55031
dc.identifier https://doi.org/10.1186/s13578-015-0009-y
dc.identifier.uri http://localhost:8080/xmlui/handle/CUHPOERS/281862
dc.description Background Aedes albopictus is an important vector of Dengue virus (DENV) and it has quickly invaded the tropical and temperate environments worldwide. A few studies have shown that, microRNAs (miRNAs) regulate mosquito defense against pathogens. However, there is no systematic analysis of the impact of DENV infection on miRNA expression in Ae. albopictus. We conducted this study to investigate the miRNA expression of Ae. albopictus upon DENV-2 infection using Illumina RNA sequencing. Results A total of 103 known and 5 novel candidate miRNAs were identified in DENV-2 infected and non-infected adult female Ae. albopictus. Comparative analysis indicated that 52 miRNAs were significantly down-regulated and 18 were up-regulated significantly after infection. Furthermore, RT-qPCR validated the expression patterns of eleven of these differentially expressed miRNAs. Targets prediction and functional analysis of these regulated miRNAs suggested that miR-34-5p and miR-87 might be involved in the anti-pathogen and immune responses. Conclusion This is the first systematic study on the impact of DENV infection on miRNA expression in Ae. albopictus. Complex changes in miRNA expression suggest a potential role of miRNAs in antiviral responses by regulating immune-related genes. This investigation provides information concerning DENV-induced miRNAs and offers clues for identifying potential candidates for vector based antiviral strategies.
dc.description Published version
dc.format application/pdf
dc.format application/pdf
dc.language en_US
dc.rights Creative Commons Attribution 4.0 International
dc.rights http://creativecommons.org/licenses/by/4.0/
dc.rights Liu et al.; licensee BioMed Central.
dc.title The expression profile of Aedes albopictus miRNAs is altered by dengue virus serotype-2 infection
dc.title Cell & Bioscience
dc.type Article - Refereed
dc.type Text


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