"Botnets" are distributed collections of compromised networked machines under common control. Botnets provide a formidable computing and communication platform by harnessing the power of thousands, or even millions, of nodes for a common collective purpose. Unfortunately, that purpose is often malicious and economically or politically motivated. This research investigates a unique approach to detecting bots, botnet infrastructure, and mitigating abusive traffic via Transport-level Traffic Analysis (TTA).